Journal: Frontiers in Endocrinology

Article Title: Association of angiotensin II and receptors in peri-implantation endometrium with microvessel density and pregnancy outcomes of women with recurrent implantation failure after embryo transfer

doi: 10.3389/fendo.2023.1206326

Figure Lengend Snippet: Ang II, AT1R, and AT2R expression in endometrium between fertile controls, RM and RIF. (A) Representative images of immunohistochemical staining of Ang II, AT1R, and AT2R among groups. LE, luminal epithelium; GE, glandular epithelium; ST, stroma cells. Scale bar=100 μm. (B) Corresponding quantitative analysis. Data are presented as mean ± SEM for AngII, AT1R, and AT2R and median (range) for stromal AT1R/AT2R expression ratios in all compartments of endometrium. *p < 0.05, ***p<0.001, NS, not significant.

Article Snippet: The slides were washed in PBS and incubated in blocking solution (5% goat serum albumin in PBS) for 1 h at room temperature, and incubated overnight at 4°C with primary antibody, either rabbit anti-human Ang II polyclonal antibody (PA5-33339, Thermo Fisher, US) at 1:400 dilution, rabbit anti-human AT1R polyclonal antibody (AAR-011, Alomone Labs, Israel) at 1:1500 dilution, rabbit anti-human AT2R antibody (ab19134, AbCam, UK) at 1:150 dilution, or rabbit polyclonal anti-human vWF antibody (A0082, DAKO, Japan) at 1:1000 dilution.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Frontiers in Endocrinology

Article Title: Association of angiotensin II and receptors in peri-implantation endometrium with microvessel density and pregnancy outcomes of women with recurrent implantation failure after embryo transfer

doi: 10.3389/fendo.2023.1206326

Figure Lengend Snippet: Arteriole and capillary density in endometrium between fertile controls, RM, and RIF. (A) Representative images of arterioles stained by AT2R and capillary stained by vWF (arrows) among groups. Scale bar = 100 μm. (B) Corresponding quantitative analysis. Data are presented as mean ± SEM. *p<0.05; NS, not significant. (C) Correlation analysis of stromal AT1R/AT2R expression ratio with arteriole density in control (r=-0.559, p=0.002), RM (r=-0.437, p=0.001) and RIF (r=-0.419, p=0.007), and its correlation with capillary density in control (r=0.256, p=0.197), RM (r=-0.227, p=0.158), and RIF (r=-0.157, p=0.334).

Article Snippet: The slides were washed in PBS and incubated in blocking solution (5% goat serum albumin in PBS) for 1 h at room temperature, and incubated overnight at 4°C with primary antibody, either rabbit anti-human Ang II polyclonal antibody (PA5-33339, Thermo Fisher, US) at 1:400 dilution, rabbit anti-human AT1R polyclonal antibody (AAR-011, Alomone Labs, Israel) at 1:1500 dilution, rabbit anti-human AT2R antibody (ab19134, AbCam, UK) at 1:150 dilution, or rabbit polyclonal anti-human vWF antibody (A0082, DAKO, Japan) at 1:1000 dilution.

Techniques: Staining, Expressing, Control

Journal: Frontiers in Endocrinology

Article Title: Association of angiotensin II and receptors in peri-implantation endometrium with microvessel density and pregnancy outcomes of women with recurrent implantation failure after embryo transfer

doi: 10.3389/fendo.2023.1206326

Figure Lengend Snippet: AT1R and AT2R expression in endometrium between women who did (Pregnant) and did not conceive (Non-pregnant) after euploid embryos transfer. (A) Representative images of immunohistochemical staining of AT1R and AT2R are shown on the left. LE=luminal epithelium; GE=glandular epithelium; ST=stroma cells. Scale bar=100 μm. Corresponding quantitative results between groups are shown on the right. Data are presented as mean ± SEM. (B) Representative images of arterioles stained by AT2R (arrows) are shown on the left. Scale bar=50 μm. Corresponding quantitative analysis between groups is shown on the right. Data are presented as mean ± SEM. (C) Comparison of the expression AT1R/AT2R ratio in all compartments of endometrium between the pregnant and non-pregnant groups is shown on the left. Data are presented as median (range). Correlation between stromal AT1R/AT2R ratio with arteriole density in the pregnant group (r=-0.438, p=0.036) and the non-pregnant group (r=-0.577, p=0.001) is shown on the right. *p<0.05, **p<0.01, ***p<0.001, NS, not significant.

Article Snippet: The slides were washed in PBS and incubated in blocking solution (5% goat serum albumin in PBS) for 1 h at room temperature, and incubated overnight at 4°C with primary antibody, either rabbit anti-human Ang II polyclonal antibody (PA5-33339, Thermo Fisher, US) at 1:400 dilution, rabbit anti-human AT1R polyclonal antibody (AAR-011, Alomone Labs, Israel) at 1:1500 dilution, rabbit anti-human AT2R antibody (ab19134, AbCam, UK) at 1:150 dilution, or rabbit polyclonal anti-human vWF antibody (A0082, DAKO, Japan) at 1:1000 dilution.

Techniques: Expressing, Immunohistochemical staining, Staining, Comparison

Journal: PLoS ONE

Article Title: The Effects of Angiotensin II and Angiotensin-(1–7) in the Rostral Ventrolateral Medulla of Rats on Stress-Induced Hypertension

doi: 10.1371/journal.pone.0070976

Figure Lengend Snippet: ACE upregulation and/or ACE2 downregulation result in Ang II↑ and/or Ang-(1–7)↓. Ang, Angiotensin; ACE, angiotensin-converting enzyme; ACE2, angiotensin-converting enzyme homolog; AT 1 R, Ang II type 1 receptor; AT 2 R, Ang II type 2 receptor; SIH, stress-induced hypertension

Article Snippet: Primary antibodies were rabbit polyclonal antibodies against AT 1 R (1∶2000) (Alomone, Israel), rabbit polyclonal antibody against AT 2 R (1∶1000) (Santa Cruz, USA) , rabbit polyclonal antibodies against Mas Receptor (1∶2000) (Alomone, Israel) , mouse monoclonal antibody against ACE (1∶1000) (abcam, USA) , goat polyclonal antibody against ACE2 (1∶1000) (Santa Cruz, USA), and mouse polyclonal antibodies against β-actin (1∶3000) (as an internal standard from Beyotime Institute of Biotechnology, Haimen, China).

Techniques:

Journal: BioMed Research International

Article Title: Gender Differences in the Progression of Experimental Chronic Kidney Disease Induced by Chronic Nitric Oxide Inhibition

doi: 10.1155/2017/2159739

Figure Lengend Snippet: Representative micrographs of immunohistochemical studies in groups Control and NAME: both male and female NAME animals showed a significant increase of renal interstitial macrophage (a) and T-cell (b) infiltration. Additionally, male NAME rats exhibited a statistically increased number of interstitial Ang II + cells, compared to gender matched Controls (c).

Article Snippet: Monoclonal mouse anti-CD3 (Dako, #M7254, ‎Glostrup, ‎Denmark) and anti- α -SMA (Sigma Chemical CO, #A2547, St. Louis, USA) and a rabbit polyclonal anti-angiotensin II (Peninsula Lab.

Techniques: Immunohistochemical staining

Journal: BioMed Research International

Article Title: Gender Differences in the Progression of Experimental Chronic Kidney Disease Induced by Chronic Nitric Oxide Inhibition

doi: 10.1155/2017/2159739

Figure Lengend Snippet: Bar graph representation of renal cortical interstitial infiltration by macrophages (a), T-cells (b), and Ang II α + cells (c) showed that both male and female NAME rats exhibited a significant increase of renal macrophage and T-cell infiltration. However, NAME females showed statistically less Ang II + interstitial cells than males subjected to the same treatment ( A p < 0.05 versus Control ♂, B p < 0.05 versus Control ♀, C p < 0.05 versus NAME ♂). The number of animals studied per group was, respectively, Control ♂: N = 5; Control ♀: N = 5; NAME ♂: N = 5; NAME ♀: N = 5.

Article Snippet: Monoclonal mouse anti-CD3 (Dako, #M7254, ‎Glostrup, ‎Denmark) and anti- α -SMA (Sigma Chemical CO, #A2547, St. Louis, USA) and a rabbit polyclonal anti-angiotensin II (Peninsula Lab.

Techniques:

Journal: BioMed Research International

Article Title: Gender Differences in the Progression of Experimental Chronic Kidney Disease Induced by Chronic Nitric Oxide Inhibition

doi: 10.1155/2017/2159739

Figure Lengend Snippet: Representative micrographs of immunofluorescence double-staining for macrophages (ED1) and angiotensin II (Ang II) in NAME male and female animals. There was no evidence of colocalization of both biomarkers in the same cell (MERGE).

Article Snippet: Monoclonal mouse anti-CD3 (Dako, #M7254, ‎Glostrup, ‎Denmark) and anti- α -SMA (Sigma Chemical CO, #A2547, St. Louis, USA) and a rabbit polyclonal anti-angiotensin II (Peninsula Lab.

Techniques: Immunofluorescence, Double Staining

Journal: BioMed Research International

Article Title: Gender Differences in the Progression of Experimental Chronic Kidney Disease Induced by Chronic Nitric Oxide Inhibition

doi: 10.1155/2017/2159739

Figure Lengend Snippet: Representative micrographs of immunofluorescence double-staining for myofibroblasts ( α -SMA) and angiotensin II (Ang II) in NAME male and female animals. There was no evidence of colocalization of both biomarkers in interstitial cells. Colocalization was only constitutively observed in glomerular arterioles and mesangial cells (MERGE).

Article Snippet: Monoclonal mouse anti-CD3 (Dako, #M7254, ‎Glostrup, ‎Denmark) and anti- α -SMA (Sigma Chemical CO, #A2547, St. Louis, USA) and a rabbit polyclonal anti-angiotensin II (Peninsula Lab.

Techniques: Immunofluorescence, Double Staining